Which stain is used as the first counterstain in Pap smear?

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Multiple Choice

Which stain is used as the first counterstain in Pap smear?

Explanation:
The staining sequence of the Papanicolaou (Pap) smear uses a nuclear stain first to define nuclei, followed by two cytoplasmic counterstains to differentiate cell types. The first cytoplasmic counterstain is orange G (OG-6). This dye selectively colors keratinized and some cytoplasmic components orange, helping to distinguish superficial keratinizing cells from deeper, non-keratinized cells. The second counterstain (EA-50) then adds additional color contrast to the cytoplasm, completing the multichromatic profile that enhances cellular morphology. So OG-6 is the correct first counterstain after the nuclear stain.

The staining sequence of the Papanicolaou (Pap) smear uses a nuclear stain first to define nuclei, followed by two cytoplasmic counterstains to differentiate cell types. The first cytoplasmic counterstain is orange G (OG-6). This dye selectively colors keratinized and some cytoplasmic components orange, helping to distinguish superficial keratinizing cells from deeper, non-keratinized cells. The second counterstain (EA-50) then adds additional color contrast to the cytoplasm, completing the multichromatic profile that enhances cellular morphology. So OG-6 is the correct first counterstain after the nuclear stain.

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