Which stain is used as the second counterstain in Pap smear?

Unlock all questions

This demo includes only 20 questions. Upgrade to access hundreds of questions, flashcards, exam simulations, and disable ads.

Full question bankExam simulationsFlashcards
From $25.99Unlock all

Study for the Histopathology and MTLE Test. Prepare with flashcards and multiple choice questions. Gain insights into the exam format, essential topics, and tips to excel your preparation!

Multiple Choice

Which stain is used as the second counterstain in Pap smear?

Explanation:
In Pap staining, you first visualize nuclei with a blue-black nuclear stain, then use cytoplasmic counterstains to differentiate cell types and maturation stages. The first cytoplasmic counterstain is orange G (OG-6), which highlights keratinized elements. The second counterstain, EA-50 (eosin azure), stains the cytoplasm of non-keratinizing cells and enhances contrast between different epithelial components, producing the characteristic multicolored appearance that helps distinguish normal from abnormal cells. Methylene blue is not part of the Pap schema, and Harris hematoxylin is the nuclear stain, not a cytoplasmic counterstain. Hence EA-50 is the second counterstain.

In Pap staining, you first visualize nuclei with a blue-black nuclear stain, then use cytoplasmic counterstains to differentiate cell types and maturation stages. The first cytoplasmic counterstain is orange G (OG-6), which highlights keratinized elements. The second counterstain, EA-50 (eosin azure), stains the cytoplasm of non-keratinizing cells and enhances contrast between different epithelial components, producing the characteristic multicolored appearance that helps distinguish normal from abnormal cells. Methylene blue is not part of the Pap schema, and Harris hematoxylin is the nuclear stain, not a cytoplasmic counterstain. Hence EA-50 is the second counterstain.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy