Masson's trichrome staining is used to highlight collagen fibers. Which statement is true regarding this staining?

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Multiple Choice

Masson's trichrome staining is used to highlight collagen fibers. Which statement is true regarding this staining?

Explanation:
Masson's trichrome is a three-color stain used specifically to differentiate connective tissue from muscle and other tissue elements, making collagen fibers stand out. In the standard protocol, collagen fibers are stained blue (by the aniline blue component), while muscle and cytoplasm take red/pink and nuclei appear dark. So the statement that Masson's trichrome staining is used to highlight collagen fibers is true. The colors red or green would not describe the collagen staining in the classic method (green can appear in some variant stains, but not in the standard Masson's trichrome).

Masson's trichrome is a three-color stain used specifically to differentiate connective tissue from muscle and other tissue elements, making collagen fibers stand out. In the standard protocol, collagen fibers are stained blue (by the aniline blue component), while muscle and cytoplasm take red/pink and nuclei appear dark. So the statement that Masson's trichrome staining is used to highlight collagen fibers is true. The colors red or green would not describe the collagen staining in the classic method (green can appear in some variant stains, but not in the standard Masson's trichrome).

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